| PCR Master Mix | x1 (Volum/µL) | x100 |
| PCR water | 14.8 | 1480 |
| 10X Buffer | 2.5 | 250 |
| 10 mM dNTPs | 0.5 | 50 |
| 20 mM Primer Forward | 1 | 100 |
| 20 mM Primer Reverse | 1 | 100 |
| Taq 5 U/µL | 0.2 | 20 |
| Total | 20 |
|
| DNA (Diluted 1:5) | 5 | From chelex extraction |
| Final volum | 25.0 |
|