PCR Master Mix | x1 (Volum/µL) | x100 |
PCR Water | 14.8 | 1480 |
Buffer10X | 2.5 | 250 |
10 mM dNTPs | 0.5 | 50 |
20 mM Primer Forward | 1 | 100 |
20 mM Primer Reverse | 1 | 100 |
Taq 5 U/µL | 0.2 | 20 |
Total | 20 |
|
DNA | 5 |
|
Final Volum | 25.0 |
|