PCR Master Mix

x1 (Volum/µL)

x100

PCR Water

14.8

1480

Buffer10X

2.5

250

10 mM dNTPs

0.5

50

20 mM Primer Forward

1

100

20 mM Primer Reverse

1

100

Taq 5 U/µL

0.2

20

Total

20

DNA

5

Final Volum

25.0