| Primer code (gene) | Pre-denaturation | Denaturation | Annealing | Elongation | ||
| Nor 1&2 (Nor(aflD) | 94˚C—10 min | 94˚C—1 min | 65˚C—1 min | 72˚C—2 min (33 cycles) | 72˚C—5 min (1 cycle | |
| Ver 1&2 (Ver(aflM) | 95˚C—4 min | 95˚C—1 min | 58˚C—1 min | 72˚C—30 sec (30 cycles) | 72˚C—10 min (1 cycle | |
| Omt 1&2 (omt) | 94˚C—5min | 94˚C—1 min | 72˚C—2 min | 72˚C—2 min (33 cycles) | 72˚C—10 min (1 cycle | |
| AflR 1&2 (aflR) | 95˚C—4 min | 95˚C—1 min | 60˚C—1 min | 72˚C—30 sec (30 cycles) | 72˚C—10 min (1 cycle | |
| AflJ F&R (aflJ) | 95˚C—10 min | 95˚C—50 sec | 58˚C—50 sec | 72˚C—2 min (30 cycles) | 72˚C—10 min (1 cycle | |