| Biochemical tests | C. perfringens type D |
| Sugar fermentation-glucose, maltose, lactose, sucrose, fructose, galactose, dulcitol | + |
| Indole production | − |
| Methyl red | − |
| Voges Proskauer | − |
| Catalase | − |
| litmus milk | + |
| Urease | − |
| H2S production | + |
| Nitrate reduction | + |
| Phospholipase reaction | + |