| Gene | Function | qPCR Primer sequence (5’-3’) | Amplicon size (nt) |
| 16s rRNA | Ribosomal RNA | q16F CAGTAGCTGGTCTGAGAGGATG q16R GTAGGAGTCTGGGCCGTGT | 62 |
| aoaA | AMDT, 1st step CYN biosynthesis | qaoaAF ACCATTCTTTGAGGTAGAGAATCAA qaoaAR AATTTCGTTCCCAAAGGTGA | 76 |
| aoaB | NRPS/PKS, 2nd step CYN biosynthesis | qaoaBF TCTCCCCAATAATCTCGCTTAC qaoaBR TGTTCCATCAAGATCCCTTTG | 75 |
| aoaC | PKS, 3rd step CYN biosynthesis | qaoaCFGGCACCTGATGGTTGTTGTA qaoaCRCTTCGCCTCGCACATAGC | 62 |
| cyrJ | Sulfotransferase, CYN biosynthesis | qcyrJF ATTTCTTTGGGTTGGCGAAT qcyrJR AGACCATGGGAATTGAGTGG | 67 |
| ntcA | Transcriptional regulator in N metabolism | qntcAF CGAAACGTTTGAACGCAATA qntcAR AGTAGACTCGTTCGGCAGGA | 62 |