Analysis Conditions |
|
Ph mobile app | A—methanol: water/8.92 (v/v) |
B—methanol: water/45.55 (v/v) | |
Gradient program | 0 - 20 20% A + 80% B |
20 - 35 15% A + 85% B | |
35 - 45 10% A + 90% B | |
45 - 50 0% A + 100% B | |
Flow rate | 1.2 ml/min |
Temperature | 20˚C |
Detection | Using UV at 288 nm |