Analysis Conditions

Ph mobile app

A—methanol: water/8.92 (v/v)

B—methanol: water/45.55 (v/v)

Gradient program

0 - 20 20% A + 80% B

20 - 35 15% A + 85% B

35 - 45 10% A + 90% B

45 - 50 0% A + 100% B

Flow rate

1.2 ml/min

Temperature

20˚C

Detection

Using UV at 288 nm