Parameters

Studied Range

Selected Value

Excitation wavelength maximum/λ_ex (nm)

200 - 700

303

Emission wavelength maximum/λ_em (nm)

200 - 700

365

Acidity/M H₂SO₄

0.001 - 0.1

0.025 - 0.1

(preferably 0.05)

pH

1.0 - 7.0

1.33 - 2.6

preferably 2.0

Time/h

0 - 72

1 min - 24 h

(preferably 2 min)

Temperature/˚C

5 - 80

10 - 70

(preferably 25 ± 5)

Reagent

(fold molar excess, M:R)

1:50 - 1:2000

1:400 - 1:1000

(preferably 1:500)

Linear range/μgL⁻¹

0.0001 - 1000

0.001 - 800

Limit of quantization/ngL⁻¹

0.01 - 100

1

Detection limit/ngL⁻¹

0.001 - 10.0

0.1

Reproducibility (% RSD)

0 - 10

0 - 2

Regression co-efficient (R²)

0.9996 - 0.9999

0.9998