Validation criteria

Conc. (µg mL⁻¹)

Artesunate

Trueness:

Absolute bias (µg mL⁻¹)

(Relative bias (%))

3000.0

7.9 (0.3)

4000.0

−4.8 (−0.1)

5000.0

15.0 (0.3)

6000.0

16.3 (0.3)

7000.0

−42.0 (−0.6)

Precision:

Repeatability (%)/

Intermediate precision (%)

3000.0

0.3/3.3

4000.0

0.2/1.3

5000.0

0.2/1.1

6000.0

0.2/1.1

7000.0

0.2/1.4

Accuracy:

95% β-expect. tol. int. (µg mL⁻¹)

[Rel. 95% β-expect. tol. int (%)]

3000.0

2680 - 3264

[−9.593, 10.13]

4000.0

3870 - 4174

[−3.9 - 3.7]

5000.0

4722 - 5084

[−3.4 - 4.0]

6000.0

5692 - 6060

[−2.9 - 3.4]

7000.0

6628 - 7211

[−4.8 - 3.6]

Linearity:

Slope

0.9916

Intercept

39.49

R²

0.9975

Dosing range:

Lower LOQ (µg mL⁻¹)

3820

Upper LOQ (µg mL⁻¹)

6962