Factor

Experiments

Test (1)

Test (2)

Test (3)

Optimization (± 5 tests)

Mobile phase:

Methanol

NH₄ formate buffer ^(*)

<50%

25%

75%

50:50%

50%

50%

>50%

75%

25%

About five more tests are added: For example at the Flow of 1.0 ± 0.3 mL min⁻¹. (0.7 mL min⁻¹ and 1.3 mL min⁻¹); 25 or 75 ± 10%, v/v of organic modifier (15:85%, or 35:65%; 65:35% or 85:15%) v/v, column dimensions, or column temperature adaptations, etc.

Flow rate (mL min⁻¹)

1.0

1.0

1.0

Column temperature (˚C)

25

25

25

Injection volume (µL)

10

10

10

Detection wavelength (nm)

210

210

210