Parameter

S-LINA

Linearity

Correlation coeffient

0.9997

Slope

76,094,169.1

Y-Intercept

1137.7

% Y-Intercept

0.99

Accuracy (% Recovery)

LOQ (n = 3)

98.6

50% (n = 3)

101.5

100% (n = 3)

100.3

150% (n = 3)

99.8

Precision (% RSD)

LOQ (n = 6)

1.53

50% (n = 6)

0.42

100% (n = 6)

0.35

Ruggedness; Different day and analyst (% RSD)

100% (n = 6)

0.49

Robustness (Resolution)

Actual flow 0.5 mL/min

5.5

Different flow 0.45 mL/min

5.8

Different flow 0.55 mL/min

5.0

Different Column Temperature 35˚C

5.2

Different Column Temperature 25˚C

5.6

Limit of Detection (Concentration in µg/mL)

0.03

Limit of Quantification (Concentration in µg/mL)

0.1