| Sr. No. | Column name and dimensions | Mobile phase composition (% v/v) | Observations and Retention time (min) | Resolution | Tailing factors of S-LINA and R-LINA |
| 1 | Chiralpak OD-H (250 × 4.6 mm) | 90:10:0.1 n-Hex:EtoH:DEA | Peaks not eluted | NA | NA |
| 30:70:0.1 n-Hex:IPA:DEA | 25.21 and 33.44 | 1.2 | 1.2 and 1.3 | ||
| 100:0.1 IPA:DEA | Peaks not eluted | No Res | NA | ||
| 100:0.1 EtoH:DEA | 20.99 and 23.47 | 1.1 | 0.9 and 1.5 | ||
| 2 | Chiralpak OJ-H (250 × 4.6 mm) | 30:70:0.1 n-Hex:IPA:DEA | 10.63 | No Res | NA |
| 100:0.1 IPA:DEA | 16.24 | No Res | NA | ||
| 100:0.1 EtOH:DEA | 11.83 | No Res | NA | ||
| 3 | Chiralpak IC (250 × 4.6 mm) | 100:0.1 EtoH:DEA | Peaks not eluted | NA | NA |
| 50:50:0.1 MeoH:EtoH:DEA | Peaks not eluted | NA | NA | ||
| 50:50:0.1 EtoH:ACN:DEA | 26.01 and 27.97 | 0.9 | 1.0 and 2.1 | ||
| 4 | Chiralpak IE (250 × 4.6 mm) | 100:0.1 EtoH:DEA | 31.59 and 34.67 | 1.2 | 1.1 and 1.4 |
| 50:50:0.1 MeoH:EtoH:DEA | Peaks not eluted | No Res | NA | ||
| 5 | Chiralpak IA (250 × 4.6 mm) | 100:0.1 IPA:DEA | 11.43 | No Res | NA |
| 100:0.1 EtoH:DEA | 7.17 and 7.87 | 1.3 | 1.1 and 1.5 | ||
| 50:50:0.1 MeoH:EtoH:DEA | 9.10 and 10.59 | 1.5 | 1.5 | ||
| 6 | Chiralpak AD-H (250 × 4.6 mm) | 90:10:0.1 n-Hex:EtoH:DEA | Peaks not eluted | NA | NA |
| 30:70:0.1 n-Hex:IPA:DEA | 10.27 | No Res | NA | ||
| 10:90:0.1 n-Hex:EtoH:DEA | 13.72 and 15.41 | 2.2 | 1.3 and 1.2 | ||
| 100:0.1 EtoH:DEA | 14.25 and 17.68 | 3.8 | 1.3 and 1.2 | ||
| 90:10:0.1 EtoH:MeoH:DEA | 14.25 and 17.69 | 5.5 | 1.1 and 1.3 |