| Time (min.) | A% | B% | Flow rate (µL/min.) |
| 0.0 | 90.0 | 10.0 | 100 |
| 2.0 | 50.0 | 50.0 | 200 |
| 5.0 | 50.0 | 50.0 | 200 |
| 5.5 | 20.0 | 80.0 | 200 |
| 7.5 | 20.0 | 80.0 | 200 |
| 8.0 | 90.0 | 10.0 | 200 |
| 9.0 | 90.0 | 10.0 | 200 |