Primers

Sequence

AT

Product size

F1 (Forward primer 1)

5’-ACGGCAGACTTCTCCTCAGGAGTCAGATGCAC-3’

63˚C

282 bp

F2 (Forward primer 2)

5’-ACGGCAGACTTCTCCTCAGGAGTCAGATGCAT-3’

63˚C

282 bp

R (Common reverse)

5’-TATCTTAGAGGGAGGGGCCTGAGGGTTT-3’