| Parameters | Notations | Experimental field | ||||
| −α | Low level (−1) | Midrange level (0) | High Level (+1) | +α | ||
| Cellulose Amount (g) | X1 | 2.76 | 5 | 7.50 | 10 | 12.24 |
| Sulfuric Acide Conc % (w/w) | X2 | 28.80 | 40 | 52.50 | 65 | 76.20 |
| Hydrolyse Time (min) | X3 | 10.00 | 10 | 50 | 90 | 125.85 |
| Hydrolyse Temperature (˚C) | X4 | 22.10 | 40 | 60 | 80 | 98.00 |
| Sonication Time (min) | X5 | 1.00 | 10 | 20 | 30 | 39.00 |