Methods

Positive rate for different concentrations (copies/μl; number of positive results/number of total samples, %)

Ct average of the lowest concentration

3.2 × 103

3.2 × 102

3.2 × 10

3.2

Our method

20/20 (100%)

20/20 (100%)

20/20 (100%)

20/20 (100%)

36.23

OIE recommended method

20/20 (100%)

20/20 (100%)

18/20 (90%)

15/20 (75%)

38.63

Commercially available kit

20/20 (100%)

20/20 (100%)

18/20 (90%)

13/20 (65%)

39.16