Serial Number

Sample Name

Concentration (copies/μl)

Ct value

Ct mean

Sample 1

Sample 2

Sample 3

Sample 4

Sample 5

1

ASFV Nucleic acid standard substance

3.2 × 10⁴

21.68

21.89

21.41

22.20

21.59

21.79

2

3.2 × 10³

25.27

25.02

25.57

25.71

ND

25.39

3

3.2 × 10²

28.39

28.42

28.33

28.71

28.45

28.46

4

3.2 × 10

31.97

31.89

32.12

32.11

31.99

32.02

5

3.2

35.32

35.82

35.19

36.24

35.26

35.56

6

Negative control

0

ND

ND

ND

ND

ND

ND