ASFV nucleic acid standard concentration | DMSO volume/total reaction volume (%) | Ct value | Ct mean ± SD | ||
Sample 1 | Sample 2 | Sample 3 | |||
320 copies/μl | 0 | 25.63 | 25.37 | 26.38 | 25.79 ± 0.52 |
1 | 25.64 | 25.76 | 24.83 | 25.41 ± 0.51 | |
1.5 | 24.34 | 24.99 | 23.55 | 24.30 ± 0.72 | |
2 | 26.60 | 25.52 | 26.02 | 26.05 ± 0.54 | |
32 copies/μl | 0 | 29.35 | 28.82 | 28.81 | 28.99 ± 0.30 |
1 | 30.76 | 28.88 | 29.27 | 29.63 ± 0.99 | |
1.5 | 27.90 | 28.40 | 28.27 | 28.19 ± 0.26 | |
2 | 29.81 | 29.36 | 31.25 | 30.14 ± 0.99 | |
3.2 copies/μl | 0 | 33.94 | 33.39 | 32.96 | 33.43 ± 0.50 |
1 | 33.39 | 34.17 | 32.88 | 33.48 ± 0.65 | |
1.5 | 32.12 | 32.68 | 31.54 | 32.11 ± 0.57 | |
2 | 35.42 | 34.89 | 34.87 | 35.06 ± 0.31 |