ASFV nucleic acid standard concentration

DMSO volume/total reaction volume (%)

Ct value

Ct mean ± SD

Sample 1

Sample 2

Sample 3

320 copies/μl

0

25.63

25.37

26.38

25.79 ± 0.52

1

25.64

25.76

24.83

25.41 ± 0.51

1.5

24.34

24.99

23.55

24.30 ± 0.72

2

26.60

25.52

26.02

26.05 ± 0.54

32 copies/μl

0

29.35

28.82

28.81

28.99 ± 0.30

1

30.76

28.88

29.27

29.63 ± 0.99

1.5

27.90

28.40

28.27

28.19 ± 0.26

2

29.81

29.36

31.25

30.14 ± 0.99

3.2 copies/μl

0

33.94

33.39

32.96

33.43 ± 0.50

1

33.39

34.17

32.88

33.48 ± 0.65

1.5

32.12

32.68

31.54

32.11 ± 0.57

2

35.42

34.89

34.87

35.06 ± 0.31