Serial Number

Sample Name

Primer/probe set

Ct value

Ct mean ± SD

Sample 1

Sample 2

1

ASFV nucleic acid standard material (3.2 × 10³ copies/μl)

Group 1

34.99

35.27

35.13 ± 0.19^a

2

Group 2

31.98

32.38

32.18 ± 0.28^b

3

Group 3

32.64

32.89

32.76 ± 0.17^b

4

Group 4

30.84

30.29

30.56 ± 0.38^c

5

Group 5

27.96

28.49

28.09 ± 0.26^d