primer | Sequence | Temperature (TM) |
Outer primers | FO 5-ACTGACCCCGGTGGCGGAGGA-3 RO5-CAGGCGTATCTGCTGGGCCTGCTC-3 | 62.1 72.1 |
112 codon inner primer | FI-I 5-GGCGCGGACATGGAGGACGgGC-3 RI-I 5-GCGGTACTGCACCAGGCGGCCtCA-3 | 73.3 73.8 |
158 codon inner primer | FI-II 5-CGATGCCGATGACCTGCAGAcGC-3 RI-II 5-CCCGGCCTGGTACACTGCCAGtCA-3 | 70 72.1 |