primer

Sequence

Temperature (TM)

Outer primers

FO 5-ACTGACCCCGGTGGCGGAGGA-3

RO5-CAGGCGTATCTGCTGGGCCTGCTC-3

62.1

72.1

112 codon inner

primer

FI-I 5-GGCGCGGACATGGAGGACGgGC-3

RI-I 5-GCGGTACTGCACCAGGCGGCCtCA-3

73.3

73.8

158 codon inner

primer

FI-II 5-CGATGCCGATGACCTGCAGAcGC-3

RI-II 5-CCCGGCCTGGTACACTGCCAGtCA-3

70

72.1