Health Audit study

Bus Santé study

Protocols

Methods

Protocols

Methods

BMI [kg/m2]*

Self-reported

BMI is calculated as weight (in kg)/height (in m2).

Body weight is measured with the subject lightly dressed, without shoes and using a medical scale (precision 0.5 kg), and standing height is measured using a medical gauge (precision 1 cm).

BMI is calculated as weight (in kg)/height (in m2). Mean of 3 measures

Waist circumference [cm]

Circumferences to within 5 mm with a centimetre tape, with the subject standing, 2 cm below belly button

Circumferences to within 1 mm with a centimetre tape, with waist mid-way between the lowest rib and the iliac crest with the subject standing at the end of gentle expiration

Systolic blood pressure [mmHg]

Arterial blood pressure is measured once in the sitting position on the right arm after at least 5 minutes rest using a standard protocol

Manual measure with sphygmomanometer (Lian Classic Spengler)

Arterial blood pressure is measured thrice in the sitting position on the right arm after at least 10 minutes rest using a standard protocol.

Validated automated oscillometric sphygmomanometer (Omron® HEM-907, Matsusaka, Japan).

Mean of 3 measures

Fasting glucose [mmol/L]

Commercially available enzymatic kits (Roche Cobas c501, CV 2.5%)

Commercially available enzymatic kits (Bayer Technicon Diagnostics, CV 1.4%)

Lipids [mmol/L]

Total cholesterol

Commercially available enzymatic kits (Roche Cobas c501, CV 3.3%)

Commercially available enzymatic kits (Bayer Technicon Diagnostics, CV 1.2%)

HDL-cholesterol

Commercially available enzymatic kits (Roche Cobas c501, CV 3.6%)

Commercially available enzymatic kits (Bayer Technicon Diagnostics, CV 1.2%)

LDL-cholesterol

Commercially available enzymatic kits (Roche Cobas c501, CV 2.3%)

Calculated using the Friedewald formula

Triglycerides

Commercially available enzymatic kits (Roche Cobas c501, CV 2.2%)

Commercially available enzymatic kits (Bayer Technicon Diagnostics, CV 1.5%)