| Gene | Primer | Nucleotide sequencing (5' to 3') | Product size (pb) | Temp (˚C) | Reference |
| katG | MtkatGf MtkatGr | ACCCGAGGCTGCTCCGCTGG CAGCTCCCACTCGTAGCCGT | 168 | 94˚C - 20 s 50˚C - 20 s 70 cycles 72˚C - 20 s | Afanas’ev MV, 2007 |
| inhA | MtfabGf MtfabGr | GCCTCGCTGGCCCAGAAAGG CTCCGGATCCACGGTGGGT | 320 | 94˚C - 20 s 56˚C - 20 s 70 cycles 72˚C - 20 s | Afanas’ev MV, 2007 |
| ahpC | ahpC1 F ahpC2 R | GCCTGGGTGTTCGTCACTGGT CGCAACGTCGACTGGCTCATA | 359 | 95˚C - 40 s 15 min (start) 94˚C - 40 s 30 cycles 57˚C - 40 s 1 min 72˚C - 40 s 15 min (final) | Valvatne H, 2009 |
| rpoB | MtrpoBf MtrpoBr | GAGGCGATCACCGCAGAC GGTACGGCGTTTCGATGAAC | 321 | 94˚C - 20 s 59˚C - 20 s 70 cycles 72˚C - 20 s | Afanas’ev MV, 2007 |
| gyrA | gyrBA-3F gyrBA-3R | AAGAGCGCCACCGACATC CAGCATCTCCATCGCCAA | 320 | 95˚C - 2 min (start) 95˚C - 30 cycles 1 min 65˚C - 1 min 72˚C - 1 min 72˚C - 10 min (final) | Liang L, 2012 |
| rrs | 16S-2F 16S-1R | CGTGGCCGTTTGTTTTGTC TGGTGCTCCTTAGAAAGGAGG |
| 95˚C - 2 min (start) 94˚C - 35 cycles 1 min 60˚C - 1 min 68˚C - 2 min 68˚C - 10 min (final) | Liang L, 2012 |