· Sodium hypochlorite (disinfectant)

· Distilled Water (dH₂O)

· 2 M MgCl₂

· Trios EDTA Sucrose (TES)

· TES/4

· Acrylamide

· Bisacrylamide

· Mercaptoethanol

· Ammonium Persulfate

· Hydrochloric acid

· 500 ml centrifuge bottle, Bottle

· Glycine

· TEMED

· Dithiothreitol(DTT)

· Tris base

· Phosphate buffered saline (1 xpbs)

· Transfer buffer

· 3% (w/v) BSA in PBS

· 0.05% (v/v) Tween 20 in 1 xpbs (PBS-T)

· 10% SDS, 100 ml

· 1.5 M Tris-HCl ph 8.8

· 1 M Tris, pH 68

· Molecular marker

· Mouse anti-his tag

· Anti mouse igg HRP

· Beckman Coulter Avanti J-E centrifuge with rotor JA-10

· Spectrophotometer

· Weight apparatus

· Electrophoresis tank

· Power supply (200 V, 0.6 A)

· Western blot cassette

· Transfer tank

· Ice packs

· Heat block

· Rocking shaker

· Magnetic stir plate

· Shallow tray

· Forceps and scissors

· Magnetic bar

· Eppendorf tubes

· Falcon tubes (15 and 50 ml)

· Nitrocellulose membrane (0.2 or 0.45 µm pore size) store in dark cool place

· Whatman 3 MM paper

· Gloves

· New Brunswick incubator (New Brunswick, England)

· His select solution (Nickle beads)

· Centrifuge

· PD10 column

· 0.5 M imidazole in PBS

· NanodropTM

· Clamp