Factor

Response

Protein yield

Amino acid yield

Net weight of wet filtrate

Supplement

−4.15 ± 2.77

0.24 ± 0.24

−0.55 ± 45.67

Mixing

2.99 ± 2.43

−0.10 ± 0.21

8.84 ± 40.06

Hydrolysis

−1.87 ± 2.77

0.52 ± 0.21

−18.75 ± 40.06