Primer

Sequence

Annealing

temperature (˚C)

Product size (bp)

ALB

F 5'-GATGAGATGCCTGCTGACTTGC-3'

R 5'-CACGACAGAGTAATCAGGATGCC-3'

60

147

AFP

F 5'-CATGAGCACTGTTGCAGAGGAGA-3'

R 5'-CGTGGTCAGTTTGCAGCATTCTG-3'

60

113

TAT

F 5'-CTTCTGGGGCTATGTACCTCA-3'

R 5'-GGACTGTGATGACCACTCGGAT-3'

55

165

CYP7A1

F 5'-CATGAGCACTGTTGCAGAGGAGA-3

R 5'-ACTCGGTAGCAGAAAGAATACATC-3'

60

388

GAPDH

F5'-CTCTCTGCTCCTCCTGTTCG-3'

R 5'-ACGACCAAATCCGTTGACTC-3'

60

142