| Gene name | Sequence | Annealing Temp/˚C | PCR product size/bp | NCBI Ref Seq. | PCR Conditions | |
| NOTCH1 | F | 5’GTGGGCTCCCGTGTTTTGTA3’ | 62 | 300 | NM_017617.5 | Initial denaturation: 95˚C for 3 min, 30 cycles of; Denaturation: 95˚C for 15 s, annealing: 62˚C for 45 s, Elongation: 72˚C for 3 min, Final Elongation: 72˚C for 10 min |
| R | 5’TCCCTCACTGGCATGACACA3’ | |||||
| CXCR7 | F | 5’ATTTGATTGCCCGCCTCAGA3’ | 62 | 149 | NM_020311.3 | |
| R | 5’GACGCTTTTGTTGGGCATGT3’ | |||||
| MYC | F | 5’TGTTGAAATGGGTCTGGGGG3’ | 62 | 129 | NM_002467.6 | Initial denaturation: 95˚C for 5 min, 30 cycles of; Denaturation: 95˚C for 30 s, annealing: 62˚C for 1 min, Elongation: 72˚C for 3 min, Final Elongation: 72˚C for 10 min |
| R | 5’TCTCACCTTCTCACCTGCCT 3’ | |||||
| FGFR1 | F | 5’ATTTCTGCCTTGGCCCTACC3’ | 62 | 175 | NM_001354369.2 | |
| R | 5’CTAGCGCAGTCTTTGGGGAA3’ | |||||
| β-Actin | F | 5’CGGCATCGTCACCAACTG3’ | 62 | 153 | NM_001101.5 | |
| R | 5’ACATGATCTGGGTCATCTTCTC3’ | |||||