| Run | Coded Units | Lactic Acid (g·l−1) | |||
| A (Peptone, g·l−1) | B (Yeast Extract, g·l−1) | C (Mineral Salts) | Observed | Predicted | |
| 1 | −1 | 0 | 1 | 37.75 | 38.27 |
| 2 | −1 | 0 | −1 | 37.23 | 34.16 |
| 3 | 0 | −1 | −1 | 41.27 | 43.18 |
| 4 | 1 | 0 | 1 | 53.52 | 56.50 |
| 5 | −1 | −1 | 0 | 36.65 | 38.15 |
| 6 | −1 | 1 | 0 | 38.63 | 41.03 |
| 7 | 0 | 1 | −1 | 47.75 | 49.69 |
| 8 | 0 | 0 | 0 | 43.72 | 42.14 |
| 9 | 1 | −1 | 0 | 53.03 | 51.16 |
| 10 | 0 | −1 | 1 | 58.47 | 57.29 |
| 11 | 1 | 0 | −1 | 39.90 | 40.80 |
| 12 | 0 | 0 | 0 | 42.04 | 42.14 |
| 13 | 0 | 1 | 1 | 55.92 | 55.38 |
| 14 | 1 | 1 | 0 | 54.18 | 52.89 |
| 15 | 0 | 0 | 0 | 40.12 | 42.14 |
| 16 | −1 | 0 | 1 | 38.94 | 38.27 |
| 17 | −1 | 0 | −1 | 37.54 | 34.16 |
| 18 | 0 | −1 | −1 | 42.15 | 43.18 |
| 19 | 1 | 0 | 1 | 53.05 | 56.50 |
| 20 | −1 | −1 | 0 | 36.14 | 38.15 |
| 21 | −1 | 1 | 0 | 40.33 | 41.03 |
| 22 | 0 | 1 | −1 | 48.29 | 49.69 |
| 23 | 0 | 0 | 0 | 42.82 | 42.14 |
| 24 | 1 | −1 | 0 | 52.39 | 51.16 |
| 25 | 0 | −1 | 1 | 59.46 | 57.29 |
| 26 | 1 | 0 | −1 | 41.55 | 40.80 |
| 27 | 0 | 0 | 0 | 43.05 | 42.14 |
| 28 | 0 | 1 | 1 | 57.79 | 55.38 |
| 29 | 1 | 1 | 0 | 55.10 | 52.89 |
| 30 | 0 | 0 | 0 | 41.07 | 42.14 |