Method

Solvent

Parameters

Pressurised liquid extraction (PLE)

acetone/cyclohexane 2/1 v/v

2 cycles, 100˚C, 140 bar, 10 min stationary phase

Soxhlet extraction (Sox)

acetone/cyclohexane 1/1 v/v (100 ml)

6 h

DIN 10382 (SLE)

1 x acetone (50 ml, 15 min),

2 x cyclohexane (50 ml, 15 min)

agitation at 300 min−1

Variants of SLE

1 x acetone (50 ml, 15 min),

1 x cyclohexane (50 ml),

1 x rinse with cyclohexane (50 ml)

length of second step varied between 1, 3, 6, or 16 h,

agitation at 300 min−1