Body weight (g) and body weight gain (g)

Body weight (g)

Body weight gain (g)

d0

d7

d0 - 7

H1¹

36.61 ± 0.29^b

126.26 ± 2.69

88.76 ± 2.57

H2

37.53 ± 0.28^a

129.00 ± 2.86

91.65 ± 2.70

SEM

0.46

1.37

1.44

p-Value

0.015

0.514

0.486

D0 yolk sac weight (g) and yolk free body weight (g)

Yolk sac (g)

YFBW² (g)

H1

3.68 ± 0.15^a

32.52 ± 0.45^b

H2

2.80 ± 0.12^b

35.08 ± 0.47^a

SEM

0.44

1.28

p-Value

<0.001

<0.001

D0 chick length and abdominal height (cm)

Length (cm)

Abdominal height (cm)

H1

18.17 ± 0.07^b

2.66 ± 0.01

H2

18.75 ± 0.05^a

2.66 ± 0.02

SEM

0.29

0.00

p-Value

<0.001

0.793

Intestinal weight (g) and spleen weight (g)

Intestinal weight (g)

Spleen weight (g)

d0

d7

d0

d7

H1

1.75 ± 0.04^b

16.06 ± 0.39

0.0709 ± 0.0275

0.1586 ± 0.0268

H2

1.98 ± 0.03^a

15.63 ± 0.41

0.0417 ± 0.0164

0.1060 ± 0.0153

SEM

0.12

0.22

0.0146

0.0263

p-Value

<0.001

0.567

0.411

0.234

Alpha-1-acid glycoprotein (μg/mL)

d0

d7

H1

159.85 ± 20.94

178.92 ± 64.13

H2

158.6 ± 24.98

314.56 ± 43.33

SEM

0.62

67.82

p-Value

0.926

0.316

Log₁₀ CFU/g aerobic recovery of liver on tryptic soy agar and Log₁₀ CFU/g Enterobacteriaceae recovery of mid-ileum and ceca on MacConkey agar

Liver (TSA)³

Mid-Ileum (MacConkey)

Ceca (MacConkey)

d0

d7

d7

d7

H1

1.85 ± 0.15^a

2.45 ± 0.12

4.28 ± 0.23

8.51 ± 0.08

H2

0.83 ± 0.13^b

2.43 ± 0.14

4.11 ± 0.24

8.40 ± 0.06

SEM

0.51

0.01

0.09

0.06

p-Value

<0.001

0.890

0.624

0.264