| Length (cm) | Lay chick on ventral side and measure chick from tip of beak to the tip of the nail on the third toe of the outstretched right leg. |
| Abdominal height (cm) | Lay chick on dorsal side and measure vertically to the highest point of the abdomen. |
| Righting (0/1) | Place chick on dorsal side and hold until the chick stops struggling, then time whether chick can right itself in less than 3 seconds. If the chick is successful, score as 0. If chick is unsuccessful in righting itself in less than 3 seconds, score as 1. |
| Navel abnormalities |
|
| Black button/ bruised (0/1) | If navel does not appear to have a black button or to be bruised, score as 0. If navel appears to have a black button or to be bruised, score as 1. |
| “String” navel (0/1) | If navel does not appear to have a “string” navel, score as 0. If navel appears to have a “string” navel, score as 1. |
| Open and unhealed (0/1) | If navel appears closed and healed, score as 0. If navel appears open or unhealed, score as 1. |
| Infected (0/1) | If navel does not appear infected, score as 0. If navel appears infected, red, or inflamed, score as 1. |
| Leg abnormalities |
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| Red hocks (0/1) | If hocks appear yellow/yellow-orange, score as 0. If hocks appear red and/or inflamed, score as 1. |
| Dehydrated legs (0/1) | If legs appear smooth and feel soft, score as 0. If legs appear scaly and feel hard/rough, score as 1. |
| Spread legs (0/1) | If the chick is able to stand upright on a smooth surface (stainless steel surface of the scale), score as 0. If the chick’s legs spread and chick is unable to stand upright on a smooth surface, score as 1. |
| Liver (0/1) | If liver is yellow to reddish brown in color, score as 0. If liver is abnormally colored, spotted, pale, or enlarged, score as 1. |
| Air sac (0/1) | If air sac is clear and transparent, score as 0. If air sac is cloudy or translucent, score as 1. |
| Yolk sac weight (g) | Remove the yolk sac and stalk, weigh, and record yolk sac weight in grams. Use yolk sac weight to calculate yolk free body weight. |
| Yolk free body weight (g) | To calculate yolk free body weight, subtract yolk sac weight from chick body weight on d0. |
| Intestinal weight (g) | Aseptically remove intestine from duodenum to cloaca, weigh, and record intestinal section weight in grams. |
| Spleen weight (g) | Remove the spleen, weigh, and record spleen weight in grams. |