Primer

Sequence * (5' a 3')

Fragment size (pb)

PCR conditions

Reference

TSrecAf

CAACTGCMYTGCGTATCGTCGAAGG

525

2 min 94˚C, 32 × (45 s 94˚C, 30 s 58˚C, 1.5 min 72˚C) and 7 min 72˚C.

[22]

TSrecAr

CGGATCTGGTTGATGAAGATCACCATG

TSatpDf

TCTGGTCCGYGGCCAGGAAG

574

2 min 94˚C, 32 × (45 s 94˚C, 30 s 58˚C, 90 s 72˚C) and 7 min 72˚C.

[22]

TSatpDr

CGACACTTCCGARCCSGCCTG

TSglnIIf

AAGCTCGAGTACATCTGGCTCGACGG

602

2 min 95˚C, 35 × (45 s 95˚C, 30 s 58˚C, 1.5 min 72˚C) and 7 min 72˚C.

[22]

TSglnIIr

SGAGCCGTTCCAGTCGGTGTCG

gyrB1043f

TTCGACCAGAAYTCCTAYAAGG

800

2 min 94˚C, 5 × (2 min 94˚C, 2 min 58˚C, 1 min 72˚C) 28 × (30 s 94˚C, 1 min 58˚C, 1 min 72˚C) and 5 min 72˚C.

[23]

gyrB1043r

AGCTTGTCCTTSGTCTGCG

dnaK1777f

AAGGARCANCAGATCCGCATCCA

370

2 min 94˚C, 32 × (1 min 94˚C, 1 min 62˚C, 40 s 72˚C), 5 min 72˚

[24] [25]

dnaK1777r

TASATSGCCTSRCCRAGCTTCAT