Oligonucleotide

Sequence (5’ to 3’)

Length (bp)

Amplicon length

(DMT)^b

^xSense (ColTqF1)

GGCCCTTAAAGGTAGTGGCG

20

95 (0)

^xSense (ColTqF2)

GCTTGGTGTTGGGGCCC

17

127 (1.1)

^xSense (ColTqF3)

GCTTGGTGTTGGGGCCCTAC

20

127 (0.6)

^yAnti-sense (ColTqR1)

GGTTTTACGGCAAGAGTCCCT

21

-

^zProbe (ColTqP1)

CCCTCCCGGAGCCTCCTTTGCGTA

24

-