N6 + Hormone (mg/l)

Observations

Callus % (AM) ± SD

Organogenesis

2,4-D

NAA

Shoot (AM) % ± SD

Root (AM) % ± SD

0

0

No callus formation but germination were observed after 30 days

0.0 ± 0.0

25.6 ± 8.2

26.7 ± 8.8

0.5

0

No callus formation but germination were observed after 30 days

0.0 ± 0.0

22.1 ± 3.7

31.1 ± 5.1

1.0

0

No callus formation and no germination were observed after 30 days

0.0 ± 0.

32.2 ± 7.7

26.7 ± 6.7

1.5

0

Whitish compacts callus found after 25 days

35.6 ± 6.9

NR

NR

2.0

0

Greenish nodular callus found after 25 days

67.8 ± 1.9

NR

NR

2.5

0

Greenish nodular callus found after 25 days

83.3 ± 8.8

NR

NR

3.0

0

Greenish nodular callus found after 25 days

82.2 ± 5.1

NR

NR

3.5

0

Yellowish compact callus found after 25 days

75.6 ± 6.9

NR

NR

4.0

0

Yellowish compact callus found after 25 days

68.9 ± 1.9

NR

NR

4.5

0

Yellowish compact callus found after 25 days

62.2 ± 9.6

NR

NR

5.0

0

Yellowish compact callus found after 25 days

60.0 ± 8.8

NR

NR

0

1.0

No callus formation observed after 30 days

0.0 ± 0.0

41.1 ± 8.4

25.6 ± 5.1

0

2.0

No callus formation observed after 30 days

0.0 ± 0.0

28.9 ± 10.2

22.2 ± 6.9

0

3.0

Whitish compacts callus found after 25 days

58.9 ± 7.7

NR

NR

0

4.0

Whitish compacts callus found after 25 days

61.1 ± 5.1

NR

NR

2.0

0.5

Greenish nodular callus found after 25 days

62.2 ± 3.9

NR

NR

2.0

1.0

Greenish nodular callus found after 25 days

76.7 ± 3.3

NR

NR

2.0

1.5

Greenish nodular callus found after 25 days

77.8 ± 6.9

NR

NR

3.0

0.5

Greenish nodular callus found after 25 days

75.6 ± 5.1

NR

NR

3.0

1.0

Yellowish compact callus found after 25 days

54.5 ± 6.9

NR

NR

3.0

1.5

Yellowish compact callus found after 25 days

51.1 ± 5.1

NR

NR