N6 + Hormone (mg/l) | Observations | Callus % (AM) ± SD | Organogenesis | ||
2,4-D | NAA | Shoot (AM) % ± SD | Root (AM) % ± SD | ||
0 | 0 | No callus formation but germination were observed after 30 days | 0.0 ± 0.0 | 25.6 ± 8.2 | 26.7 ± 8.8 |
0.5 | 0 | No callus formation but germination were observed after 30 days | 0.0 ± 0.0 | 22.1 ± 3.7 | 31.1 ± 5.1 |
1.0 | 0 | No callus formation and no germination were observed after 30 days | 0.0 ± 0. | 32.2 ± 7.7 | 26.7 ± 6.7 |
1.5 | 0 | Whitish compacts callus found after 25 days | 35.6 ± 6.9 | NR | NR |
2.0 | 0 | Greenish nodular callus found after 25 days | 67.8 ± 1.9 | NR | NR |
2.5 | 0 | Greenish nodular callus found after 25 days | 83.3 ± 8.8 | NR | NR |
3.0 | 0 | Greenish nodular callus found after 25 days | 82.2 ± 5.1 | NR | NR |
3.5 | 0 | Yellowish compact callus found after 25 days | 75.6 ± 6.9 | NR | NR |
4.0 | 0 | Yellowish compact callus found after 25 days | 68.9 ± 1.9 | NR | NR |
4.5 | 0 | Yellowish compact callus found after 25 days | 62.2 ± 9.6 | NR | NR |
5.0 | 0 | Yellowish compact callus found after 25 days | 60.0 ± 8.8 | NR | NR |
0 | 1.0 | No callus formation observed after 30 days | 0.0 ± 0.0 | 41.1 ± 8.4 | 25.6 ± 5.1 |
0 | 2.0 | No callus formation observed after 30 days | 0.0 ± 0.0 | 28.9 ± 10.2 | 22.2 ± 6.9 |
0 | 3.0 | Whitish compacts callus found after 25 days | 58.9 ± 7.7 | NR | NR |
0 | 4.0 | Whitish compacts callus found after 25 days | 61.1 ± 5.1 | NR | NR |
2.0 | 0.5 | Greenish nodular callus found after 25 days | 62.2 ± 3.9 | NR | NR |
2.0 | 1.0 | Greenish nodular callus found after 25 days | 76.7 ± 3.3 | NR | NR |
2.0 | 1.5 | Greenish nodular callus found after 25 days | 77.8 ± 6.9 | NR | NR |
3.0 | 0.5 | Greenish nodular callus found after 25 days | 75.6 ± 5.1 | NR | NR |
3.0 | 1.0 | Yellowish compact callus found after 25 days | 54.5 ± 6.9 | NR | NR |
3.0 | 1.5 | Yellowish compact callus found after 25 days | 51.1 ± 5.1 | NR | NR |