Action item | Number | |||||
I-1 | I-2 | I-3 | II-1 | II-2 | II-3 | |
Amylase stock solution/ml | 1.0 | 1.0 | 1.0 | 0 | 0 | 0 |
Passivate β-amylase | Heat the supernatant for 15 min at 70˚C | |||||
Amylase stock solution/ml | 0 | 0 | 0 | 1.0 | 1.0 | 1.0 |
3,5-dinitrosalicylic acid/ml | 2.0 | 0 | 0 | 2.0 | 0 | 0 |
Pre heat preservation | Heat the mixture for 10 min at 40˚C | |||||
1% starch solution/ml (40˚C) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
Heat preservation | Heat the mixture for 5 min at 40˚C | |||||
3,5-dinitrosalicylic acid/ml | 0.0 | 2.0 | 2.0 | 0.0 | 2.0 | 2.0 |