Amplified fragments for poplar transformation | Primer |
PtABA3 promoter | Forward: GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT ATA TGC CTC TGT ACA TCG TGT G |
Reverse: GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC ATG TTC TTT TTG GCT CTT CTT | |
PtAO2 promoter outer primer for nested PCR | Forward: GGA AGT CAA TTG GCT GTT GAC ATT |
Reverse: CTG TGT TTT TTG TGT CTG CGT CAG | |
PtAO2 promoter inner primer for nested PCR | Forward: AAC CCA TCC AAC TTG ATG GCC ACC |
Reverse: TTG GAT CCA CAG CGA GCG CAT AAG AAG CTG GC | |
ABA3-RNAi with intron | Forward: TTG GAT CCT CGA GTT CCT GAC ATG TGC TCT ATT AGT |
Reverse: TTC CAT GGA ATT CAA TCT GTT GTT GAG GTC GGA CAC | |
ABA3-RNAi fusion PCR | Forward: ACT GAT TTT GAA GAG AAT GAT GTC GAC TTG TGG |
Reverse: GAC ATC ATT CTC TTC AAA ATC AGT ATT ACC ATA CC | |
AO-RNAi | Forward: AAG GAT CCT CGA GTG CCT GTT GTG CAT GAA G |
Reverse: AAC CAT GGA ATT CAA GTC AGG GAC AAA GAA AG | |
GFP-ABA3 | Forward: TTT CTT GGA TCC ATG GAA GCA TTT CTT AAG GAA TTC Reverse: CAC AAG CGG ATC CTT ATT CAA TAT CTG GAT TAA CTT CTT CCC C |