S. NO.

Primer name

Sequence [5’-3’]

MOL.WT.

GC contents (%)

TM ˚C

1

A-09

GGGTAACGCC

3053.0

70.0

34.0˚C

2

A-18

AGGTGACCGT

3068.0

60.0

32.0˚C

3

B-08

GTCCACACGG

3013.0

70.0

34.0˚C

4

B-18

CCACAGCAGT

2997.0

60.0

32.0˚C

5

B-19

ACCCCCGAAG

2981.9

70.0

34.0˚C