Factor No.

Factor name

Level

1

2

3

Factor A

The type of liquid processed

Purified water

Redistilled aromatic water

Redistilled aromatic water after refrigeration and filtration

Factor B

The type of solubilizer

Tween 80 (0.25%)

Hydroxypropyl β-cyclodextrin (0.4%)

No solubilizer added

Factor C

The dosage of activated carbon

0.05%

no activated carbon added

0.10%

Factor D

Heating temperature

80˚C, 5 min

100˚C, 5 min

No heating process

Factor E

Reagents for pH adjustment

Hydrochloric acid/sodium

hydroxide

Citric acid/sodium citrate

Hydrochloric acid/sodium hydroxide

Factor F

pH value range after adjustment

4.5 - 4.8

5.5 - 5.8

7.5 - 7.8

Factor G

Sterilization conditions

100˚C, 30 min

115˚C, 30 min

121˚C, 13 min