Mutation

Restriction enzyme

Cutting position

MTHFR C677T

Hin fI

G ANTC

CTNA G

MTHFR 1298

MboII

GAAGA(N)8

CTTCT(N)7

V G1691AF

HindIII

A AGCTT

TTCGA A

FII G20210A

MnlI

CCTC(N)7

GGAG(N)6