|
| The nucleotide sequence of primer | Annealing temperature | size of amplified fragment |
| MTHFR C677T | F-TGAAGGAGAAGGTGTCTGCGGGA R-AGGACGGTGCGGTGAGAGTG | 59˚C 58˚C | 198 bp |
| MTHFR A1298C | F-CTTTGGGGAGCTGAAGGACTACTAC R-CACTTTGTGACCATTCCGGTTTG | 59˚C 55˚C | 163 bp |
| FV G1691A | F-CATGAGAGACATCGCCTCTG R-GACCTAACATGTTCTAGCCAGAAG | 54˚C 56˚C | 147 bp |
| FII G20210A | F-TCTAGAAACAGTTGCCTGGC R-ATAGCACTGGGAGCATTGAAGC | 52˚C 55˚C | 345 bp |