The nucleotide sequence of primer

Annealing temperature

size of amplified fragment

MTHFR C677T

F-TGAAGGAGAAGGTGTCTGCGGGA

R-AGGACGGTGCGGTGAGAGTG

59˚C

58˚C

198 bp

MTHFR A1298C

F-CTTTGGGGAGCTGAAGGACTACTAC

R-CACTTTGTGACCATTCCGGTTTG

59˚C

55˚C

163 bp

FV

G1691A

F-CATGAGAGACATCGCCTCTG

R-GACCTAACATGTTCTAGCCAGAAG

54˚C

56˚C

147 bp

FII

G20210A

F-TCTAGAAACAGTTGCCTGGC

R-ATAGCACTGGGAGCATTGAAGC

52˚C

55˚C

345 bp