| Primer ID Sequence (5'-3') Tm (˚C) Primer_Quality (%) PCR_Fragment_Size (bp) Topt (˚C) |
| 2F7_1_68-87 CTGGGCTGAACTAACAAGCTC 59.9 90 2R5_1_262-280 AGAAAGCTCACGGTTCCCTC 57.3 97 214 601 |
| 2F12_1_209-230 CTGTGATCTCTCCGCCATTACT 56.5 88 2R5_1_262-280 AATTCGGCTGCCACGAGTT 57.3 97 73 61 |
| 2F9_1_179-198 GGAGTTGTTTCTGGCAATGC 56.2 87 2R5_1_262-280 AATTCGGCTGCCACGAGTT 57.3 97 103 61 |
| 2F2_1_4-24 CGCCATGATTTGGACCATCGT 58.4 85 2R5_1_262-280 AATTCGGCTGCCACGAGTT 57.3 97 278 63 |
| 2F14_1_227-247 TACTAAGCCCTGGCCTATTCA 54.8 85 2R5_1_262-280 AATTCGGCTGCCACGAGTT 57.3 97 55 59 |
| 2F17_1_251-268 CGCCGATCTGGGCTGAACTA 52.6 92 2R4_1_282-299 TGCATTGACCATGTCTCTCGTT 52.5 88 50 402 |
| 2F17_1_251-268 ACGGATAGCAGAACTCGT 52.6 92 2R2_1_330-347 GGGCCTCAGCGTCTATGA 57.0 86 98 57 |
| 2F7_1_68-87 GCCGATCTGGGCTGAACTAAC 59.9 90 2R7_1_248-268 GCTCACGGTTCCCTCAGAAT 55.6 88 202 602 |
| 2F1_1_2-21 GGCGCCATGATTTGGACCAT 59.1 79 2R5_1_262-280 AATTCGGCTGCCACGAGTT 57.3 97 280 63 |
| 2F12_1_209-230 CTGTGATCTCTCCGCCATTACT 56.5 88 2R4_1_282-299 GATCAGCGGCATGTTCTA 52.5 88 92 57 |