| Primer ID Sequence (5'-3') Tm (˚C) Primer_Quality (%) PCR_Fragment_Size (bp) Topt (˚C) |
| 1F3_1_72-89 CTGGGCTGAACTAACAAGCTC 53.0 92 1R2_1_316-333 AGAAAGCTCACGGTTCCCTC 50.2 89 263 601 |
| 1F3_1_72-89 TTACCGACTTCAGCGGAT 53.0 92 1R21_1_156-174 CACTGAGCCGTGTTAAAGC 55.3 87 104 58 |
| 1F3_1_72-89 TTACCGACTTCAGCGGAT 53.0 92 1R22_1_137-156 CTTTGTGGTAGGCTCCGTCT 57.0 87 86 57 |
| 1F3_1_72-89 TTACCGACTTCAGCGGAT 53.0 92 1R6_1_293-310 GTGCTCCATGGCAGTACA 55.3 87 240 59 |
| 1F3_1_72-89 TTACCGACTTCAGCGGAT 53.0 92 1R23_1_119-136 TCTTTCCATCGGCAAAGA 51.3 87 66 56 |
| 1F3_1_72-89 GCCGATCTGGGCTGAACTAAC 53.0 92 1R13_1_223-242 GCTCACGGTTCCCTCAGAAT 57.7 87 172 602 |
| 1F3_1_72-89 TTACCGACTTCAGCGGAT 53.0 92 1R7_1_284-303 ATGGCAGTACAGCCAAGGTC 57.8 87 233 58 |
| 1F3_1_72-89 TTACCGACTTCAGCGGAT 53.0 92 1R11_1_247-266 AATCATGGCTTAAAGCGGCA 55.3 87 196 58 |
| 1F22_1_256-277 AAGCCATGATTTGGACCATCGT 56.9 90 1R5_1_295-316 TTGATGGTGCTCCATGGCAGTA 58.3 88 62 61 |
| 1F22_1_256-277 CGCCGATCTGGGCTGAACTA 56.9 90 1R6_1_293-310 TGCATTGACCATGTCTCTCGTT 55.3 87 56 402 |