Heat treatment conditions

Untreated fresh milk

Heat-treated milk samples at different temperatures and time

Uncontrolled heat treatment

Mean AFM1 conc. of initial samples (M1)

AFM1 conc. of M2

AFM1 conc. of M3

AFM1 conc. of M4

AFM1 conc. of M5

AFM1 conc. of M6

AFM1 conc. of M7

No heat treatment

0.24 ± 5.2^a

61˚C for 15 min

-

0.21^a

61˚C for 20 min

-

-

0.21^a

80˚C for 20 min

-

-

-

0.19^a

100˚C for 20 min

-

-

-

-

0.20^a

-

121˚C for 15 min

-

-

-

-

-

0.13^b

-

Uncontrolled heat treatment for 20 min

-

-

-

-

-

-

0.19^a