Volumes	Positive (+)		Positive (++)		Positive (+++)		Positive (++++)		Negative (−)
Volumes	25 µL	50 µL	25 µL	50 µL	25 µL	50 µL	25 µL	50 µL	25 µL	50 µL
Operator 1	206	206	225	225	178	178	160	160	231^*	231^*
Operator 2	206	206	225	225	178	178	160	160	231^*	231^*