Gene

Primers (5'→3')

Amplicon (pb)

Tm (˚C)

gelE

F: CGAAGTTGGAAAAGGAGGC

333

56

R: GGTGAAGAAGTTACTCTGA

esp

F: AGATTTCATCTTTGATTCTTGG

188

R: AATTGATTCTTTAGCATCTGG

asa1

F: AAGAAAAAGAAGTAGACCAAC

261

R: AAACGGCAAGACAAGTAAATA