Serum samples

Number

Visualization of amplification products by

Turbidity

Color change of HNB dye

Amplification curve in real-time thermal cycler

Step-ladder pattern band in agarose gel electrophoresis

CHIKV IgM +ve

70

70 (100%)

70 (100%)

70 (100%)

70 (100%)

CHIKV IgM –ve and IgG +ve

20

00(00%)

00(00%)

00(00%)

00(00%)

CHIKV IgM and IgG both –ve

20

00(00%)

00(00%)

00(00%)

00(00%)

Healthy controls

10

00(00%)

00(00%)

00(00%)

00(00%)