| Serum samples | Number | Number(%) positive for CHIKV RNA using | |||
| Heat block | Water bath | Conventional thermal cycler | Real-time thermal cycler | ||
| CHIKV IgM +ve | 70 | 70 (100%) | 70 (100%) | 70 (100%) | 70 (100%) |
| CHIKV IgM −ve and IgG +ve | 20 | 00 (00%) | 00 (00%) | 00 (00%) | 00 (00%) |
| CHIKV IgM −ve and IgG −ve | 20 | 00 (00%) | 00 (00%) | 00 (00%) | 00 (00%) |
| Healthy control | 10 | 00 (00%) | 00 (00%) | 00 (00%) | 00 (00%) |