Component | Volume |
1. 5× Hot Fire pol Eva Green qPCR mix plus | 4 µL |
2. Forward primer (10 µM) | 0.5 µL |
3. Backward primer (10 µM) | 0.5 µL |
4. cDNA template | 5 µL |
5. PCR grade water | 10 µL |
6. Total | 20 µL |