Run

X₁

Molasses (g/L)

X₂

Nitrate (g/L)

X₃

Inoculum size (ml)

X₄

Medium volume (ml)

Response (Y)

E24

1

15(0)

15(0)

2(0)

60(2)

79.4

2

10(−1)

20(1)

1.5(−1)

50(1)

58

3

15(0)

15(0)

2(0)

40(0)

73.2

4

10(−1)

20(1)

1.5(−1)

30(−1)

81.8

5

20(1)

10(−1)

2.5(1)

50(1)

85.6

6

20(1)

20(1)

1.5(−1)

30(−1)

69.3

7

15(0)

5(−2)

2(0)

40(0)

80

8

10(−1)

20(1)

1.5(−1)

30(−1)

73

9

15(0)

15(0)

2(0)

40(0)

86.7

10

20(1)

10(−1)

1.5(−1)

50(1)

88.3

11

15(0)

15(0)

2(0)

40(0)

77

12

15(0)

25(2)

2(0)

40(0)

78.8

13

15(0)

15(0)

2(0)

40(0)

71

14

15(0)

15(0)

1(−2)

40(0)

51

15

10(−1)

10(−1)

2.5(1)

50(1)

55.3

16

20(1)

20(1)

2.5(1)

50(1)

78.4

17

15(0)

15(0)

2(0)

40(0)

73.2

18

10(−1)

10(−1)

2.5(1)

30(−1)

55

19

10(−1)

10(−1)

2.5(1)

50(1)

70

20

25(2)

15(0)

2(0)

40(0)

93.8

21

10(−1)

20(1)

2.5(1)

50(1)

52.8

22

15(0)

15(0)

2(0)

40(0)

72

23

20(1)

20(1)

2.5(1)

30(−1)

60

24

20(1)

10(−1)

2.5(1)

30(−1)

60

25

15(0)

15(0)

3(2)

40(0)

60

26

10(−1)

10(−1)

1.5(−1)

30(−1)

53

27

20(1)

20(1)

1.5(−1)

50(1)

95

28

15(0)

15(0)

2(0)

40(0)

71

29

5(−2)

15(0)

2(0)

40(0)

55

30

15(0)

15(0)

2(0)

20(−2)

65.2

31

20(1)

10(−1)

1.5(−1)

20(−2)

59.3