| Multiplex groups | Targeted genes | Primers (5’-3’) | Size (pb) | Targeted species |
| Group 1 | curA-F | GTAAAAGAATTAAGTATGACA | 180 | L. curvatus |
| curA-R | TTACATTCCAGCTAAACCACT | |||
| sakP-F | ATGGAAAAGTTTATTGAATTA | 200 | L. sakei | |
| sakP-R | TTATTTATTCCAGCCAGCGTTTC | |||
| entB-F | GAAAATGATCACAGAATGCCTA | 162 | Enterococcus faecium | |
| entB-R | GTTGCATTTAGAGTATACATTTG | |||
| entA-F | AAATATTATGGAAATGGAGTGTAT | 126 | E. faecium | |
| entA-R | GCACTTCCCTGGAATTGCTC | |||
| plnEF-F | GGCATAGTTAAAATTCCCCCC | 428 | L. plantarum | |
| plnEF-R | CAGGTTGCCGCAAAAAAAG | |||
| Group 2 | entA-F | AAATATTATGGAAATGGAGTGTAT | 126 | E. faecium |
| entA-R | GCACTTCCCTGGAATTGCTC | |||
| entP-F | TATGGTAATGGTGTTTATTGTAAT | 112 | E. faecium | |
| entP-R | ATGTCCCATACCTGCCAAAC | |||
| pedA-F | AAAATATCTAACTAATACTTG | 600 | Pediococcus acidilactici | |
| pedA-R | TAAAAAGATATTTGACCAAAA | |||
| sakQ-F | ATGCAAAATACAAAAGAACTAA | 200 | L. sakei | |
| sakQ-R | CGCTTGTTTAGAGACACCCGTT | |||
| plnA-F | GTACAGTACTAATGGGAG | 450 | L. plantarum | |
| plnA-R | CTTACGCCAATCTATACG |