Multiplex groups

Targeted genes

Primers (5’-3’)

Size (pb)

Targeted species

Group 1

curA-F

GTAAAAGAATTAAGTATGACA

180

L. curvatus

curA-R

TTACATTCCAGCTAAACCACT

sakP-F

ATGGAAAAGTTTATTGAATTA

200

L. sakei

sakP-R

TTATTTATTCCAGCCAGCGTTTC

entB-F

GAAAATGATCACAGAATGCCTA

162

Enterococcus faecium

entB-R

GTTGCATTTAGAGTATACATTTG

entA-F

AAATATTATGGAAATGGAGTGTAT

126

E. faecium

entA-R

GCACTTCCCTGGAATTGCTC

plnEF-F

GGCATAGTTAAAATTCCCCCC

428

L. plantarum

plnEF-R

CAGGTTGCCGCAAAAAAAG

Group 2

entA-F

AAATATTATGGAAATGGAGTGTAT

126

E. faecium

entA-R

GCACTTCCCTGGAATTGCTC

entP-F

TATGGTAATGGTGTTTATTGTAAT

112

E. faecium

entP-R

ATGTCCCATACCTGCCAAAC

pedA-F

AAAATATCTAACTAATACTTG

600

Pediococcus acidilactici

pedA-R

TAAAAAGATATTTGACCAAAA

sakQ-F

ATGCAAAATACAAAAGAACTAA

200

L. sakei

sakQ-R

CGCTTGTTTAGAGACACCCGTT

plnA-F

GTACAGTACTAATGGGAG

450

L. plantarum

plnA-R

CTTACGCCAATCTATACG