| Oligonucleotide name | Oligonucleotide 5’-3’ | Target gene | PCR annealing temperature | Amplification product (bp) | Accession number/reference |
|
| Resistance genes |
|
|
|
|
| blaTEM | ATGAGTATTCAACAT TTC CG-F CCAATGCTTAATCAG TGA-R | blaTEM | 53.5 | 840 | EF125012 |
| cmy | ATGATGAAAAAATCGTTATGC-F TTGCAGCTTTTCAAGAATGCG-R | BlaCMYs | 55 | 1200 | U77414 |
| Ctx-m | ATGTGCAGYACCAGTAARGTK-F ATGGCRAARTARGTSACCAGA-R | BlaCTX-M | 60 | 593 | Y10278 |
| eae | CTGAACGGCGATTACGCGAA-F CGAGAGACGATACGATCCAG-R | eae | 54 | 917 | [8] |
| bfp | AATGGTGCTTGCGCTTGCTGC-F GCCGCTTTATCCAACCTGGTA-R | bfp | 54 | 326 | [8] |
|
| Virulence genes |
|
|
|
|
| Aggr | GTATACACAAAGAAGGAAGC-F ACAGAATCGTCAGCATCAGC-R | Aggrks a1, Aggrks a2 | 54 | 254 | [8] |
| lt | GCACACGGAGCTCCTCAGTC-F TCCTTCATCCTTTCAATGGCTTT-R | lt | 54 | 218 | [9] |
| st | GCTAAACCAGTAGASTCTTCAAAA-F CCCGGTACARGCAGGATTACAACA-R | st | 54 | 147 | [10] |
| Ipa H | CTCGGCACGTTTTAATAGTCTGG-F GTGGAGAGCTGAAGTTTCTCTGC-R | Ipa H | 54 | 933 | [9] |
| Inv | ATATCTCTATTTCCAATCGCGT-F GATGGCGAGAAATTATATCCCG-R | Inv | 54 | 382 | [11] |
| Int 1 | TCGGTCAAGGTT-F AACTTTCAGCACATG-R | Int 1 | 50 | 923 | U12338 |