| Species | GenBank ITS accession no. | Forward primer | Reverse primer | Molecular target size (bp) | Electrophoretic size (± ~0.5 bp) | Color |
| M. tuberculosis complex§§¹ | AJ315568 | IS6110 f1 IS6110 f2 | IS6110 r1 IS6110 r2 | 148 | 145 | Blue |
| M. avium complex§§/M. malmoense§§² | AY701785 | MAC f | MAC r | 103 | 103 | Green |
| M. scrofulaceum§§ | AB026702 | SCRO f | MAC r | 104 | 105 | Blue |
| M. fortuitum§ | AF144326 | MFG f | MCAG r | 134 | 137.5 | Green |
| M. kansasii§§ | DQ134002 | MKG f | MAC r | 106 | 104 | Black |
| M. ulcerans§§/M. marinum§§ | AB548734 AB026701 | MKG f | MAC r | 106 | 106 | |
| M. chelonae§ | AF144327 | MKG f | MCAG r | 91 | 90 | Black |
| M. haemophilum§§/M. terrae§§3 | DQ851570 | MKG f | MAC r | 120/121 | 120/121 | Black |
| M. abscessus§ | AJ314870 | MKG f | ABSC r | 91 | 92 | Black |
| M. gordonae§§ | FJ858761 | MKG f | MAC r | 96 | 96 | Black |
| M. xenopi§§ | L15624 | MKG f | MCAG r | 95 | 94 - 96 (4 peaks) | Black |
| M. mucogenicum§ | AY504951 | MUCOG f | MCAG r | 151 | 154 + 158 | Green |
| β-globin. control | NG000007 | β-globin f | β-globin r | 153 | 151 | Blue |